Evaluation of the optimal multiplex PCR method for the detection of aspergillus flavus and aspergillus parasiticus on dried peanut

Aspergillus flavus and Aspergillus parasiticus areq the common aflatoxin producing species that usually infect onq foodstuff in their production line from the field to the storageq place such as peanut, corn, cereal, etc., especially in tropicalq country like Vietnam. Aflatoxins which are considered as derivedq secondary metabolites assigned as a group of mycotoxinsq produced by several species of the Aspergillus spp are potentq hepatotoxins, immunosuppression, carcinogen that lead toq mortality or reducing the productivity of farm animals. Thereq has been a demand for effective method to detect these twoq species on dried food. In previous study, a multiplex PCR methodq were designed to improve the detection process of A. flavusq and A. parasiticus and that method showed high sensitivityq and specificity by being applied on artificially infected driedq peanut. In this study, that multiplex PCR method would beq evaluated by testing the presence of A. flavus and A. parasiticusq on natural dried peanut kernels. On this purpose, the presenceq of A. flavus and A. parasiticus on the collected peanut from theq market was determined using two method, the conventionalq culturing method in Institute of Hygiene and Public Healthq (IHPH) and the mentioned multiplex PCR. The efficiency ofq multiplex PCR method would be evaluated by comparing fungiq detection result of two methods using appropriate statisticalq tests. Next, fungal enrichment with distilled water overnightq was applied to increase the detection percentage if the firstq analysis do not get the expected result. The result showed thatq 54% results from PCR method was the same with culturingq method, and after fungal enrichment, this percentage increasedq to 76% which suggested that these two method was notq significantly different with each other. Therefore, this multiplexq PCR method could have more advanced points than theq culturing method in detection of A. flavus and A. parasiticus onq foodstuff.

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